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anti fkbp12 6 antibody  (Proteintech)


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    Structured Review

    Proteintech anti fkbp12 6 antibody
    Anti Fkbp12 6 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fkbp12+6/FKBP1B+Antibody/pm40943170-158-13-16
    Average 93 stars, based on 3 article reviews
    anti fkbp12 6 antibody - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial Damage.
    Article Snippet: Proteins (8–12% SDS-PAGE) were separated and transferred onto PVDF membranes (Beyotime, FFP33/FFP24, Haimen, China) using a semi-dry transfer system (Trans-Blot Turbo). .. Membranes were blocked with 5% skim milk for 1 h, then incubated overnight at 4 ◦C with primary antibodies: OPA1 (1:1000, Proteintech, 27733-1-AP, Wuhan, China), PGC-1α (1:1000, Abclona, A12348), MFN1 (1:1000, Proteintech, 13798-1-AP), β-Tubulin (1:2000, Servicebio, GB11017-100), β-actin (1:1000, Servicebio, GB15003-100), GAPDH (1:5000, Servicebio, GB15004-100), LC3 (1:500, Proteintech, 14600-1-AP), Fis1 (1:1000, Proteintech, 10956-1-AP), TOM-20 (1:4000, Proteintech, 11802-1-AP), Cyt-C (1:1000, Abcam, ab133504), SERCA2 (1:1000, Proteintech, 27311-1-AP), MCU (1:1000, Proteintech, 26312-1-AP), PPID (1:500, Proteintech, 12716-1-AP), FKBP12.6 (1:2000, Proteintech, 15114-1-AP), GLMN (1:500, Proteintech, 32645-1-AP), and NR3C1 (1:1000, Proteintech, 24050-1-AP). ..

    Article Title: NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial Damage
    Article Snippet: Proteins (8–12% SDS-PAGE) were separated and transferred onto PVDF membranes (Beyotime, FFP33/FFP24, Haimen, China) using a semi-dry transfer system (Trans-Blot Turbo). .. Membranes were blocked with 5% skim milk for 1 h, then incubated overnight at 4 °C with primary antibodies: OPA1 (1:1000, Proteintech, 27733-1-AP, Wuhan, China), PGC-1α (1:1000, Abclona, A12348), MFN1 (1:1000, Proteintech, 13798-1-AP), β-Tubulin (1:2000, Servicebio, GB11017-100), β-actin (1:1000, Servicebio, GB15003-100), GAPDH (1:5000, Servicebio, GB15004-100), LC3 (1:500, Proteintech, 14600-1-AP), Fis1 (1:1000, Proteintech, 10956-1-AP), TOM-20 (1:4000, Proteintech, 11802-1-AP), Cyt-C (1:1000, Abcam, ab133504), SERCA2 (1:1000, Proteintech, 27311-1-AP), MCU (1:1000, Proteintech, 26312-1-AP), PPID (1:500, Proteintech, 12716-1-AP), FKBP12.6 (1:2000, Proteintech, 15114-1-AP), GLMN (1:500, Proteintech, 32645-1-AP), and NR3C1 (1:1000, Proteintech, 24050-1-AP). ..

    Pyrolysis Gas Chromatography:

    Article Title: NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial Damage.
    Article Snippet: Proteins (8–12% SDS-PAGE) were separated and transferred onto PVDF membranes (Beyotime, FFP33/FFP24, Haimen, China) using a semi-dry transfer system (Trans-Blot Turbo). .. Membranes were blocked with 5% skim milk for 1 h, then incubated overnight at 4 ◦C with primary antibodies: OPA1 (1:1000, Proteintech, 27733-1-AP, Wuhan, China), PGC-1α (1:1000, Abclona, A12348), MFN1 (1:1000, Proteintech, 13798-1-AP), β-Tubulin (1:2000, Servicebio, GB11017-100), β-actin (1:1000, Servicebio, GB15003-100), GAPDH (1:5000, Servicebio, GB15004-100), LC3 (1:500, Proteintech, 14600-1-AP), Fis1 (1:1000, Proteintech, 10956-1-AP), TOM-20 (1:4000, Proteintech, 11802-1-AP), Cyt-C (1:1000, Abcam, ab133504), SERCA2 (1:1000, Proteintech, 27311-1-AP), MCU (1:1000, Proteintech, 26312-1-AP), PPID (1:500, Proteintech, 12716-1-AP), FKBP12.6 (1:2000, Proteintech, 15114-1-AP), GLMN (1:500, Proteintech, 32645-1-AP), and NR3C1 (1:1000, Proteintech, 24050-1-AP). ..

    Article Title: NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial Damage
    Article Snippet: Proteins (8–12% SDS-PAGE) were separated and transferred onto PVDF membranes (Beyotime, FFP33/FFP24, Haimen, China) using a semi-dry transfer system (Trans-Blot Turbo). .. Membranes were blocked with 5% skim milk for 1 h, then incubated overnight at 4 °C with primary antibodies: OPA1 (1:1000, Proteintech, 27733-1-AP, Wuhan, China), PGC-1α (1:1000, Abclona, A12348), MFN1 (1:1000, Proteintech, 13798-1-AP), β-Tubulin (1:2000, Servicebio, GB11017-100), β-actin (1:1000, Servicebio, GB15003-100), GAPDH (1:5000, Servicebio, GB15004-100), LC3 (1:500, Proteintech, 14600-1-AP), Fis1 (1:1000, Proteintech, 10956-1-AP), TOM-20 (1:4000, Proteintech, 11802-1-AP), Cyt-C (1:1000, Abcam, ab133504), SERCA2 (1:1000, Proteintech, 27311-1-AP), MCU (1:1000, Proteintech, 26312-1-AP), PPID (1:500, Proteintech, 12716-1-AP), FKBP12.6 (1:2000, Proteintech, 15114-1-AP), GLMN (1:500, Proteintech, 32645-1-AP), and NR3C1 (1:1000, Proteintech, 24050-1-AP). ..

    Ubiquitin Proteomics:

    Article Title: NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial Damage
    Article Snippet: .. The ubiquitination level of FKBP12.6 was assessed by Western blot using a specific anti-FKBP12.6 antibody (1:2000; Proteintech, 15114-1-AP). .. All data were analyzed using GraphPad Prism (version 9).

    Article Title: NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial Damage.
    Article Snippet: .. The ubiquitination level of FKBP12.6 was assessed by Western blot using a specific anti-FKBP12.6 antibody (1:2000; Proteintech, 15114-1-AP). .. All data were analyzed using GraphPad Prism (version 9).

    Western Blot:

    Article Title: NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial Damage
    Article Snippet: .. The ubiquitination level of FKBP12.6 was assessed by Western blot using a specific anti-FKBP12.6 antibody (1:2000; Proteintech, 15114-1-AP). .. All data were analyzed using GraphPad Prism (version 9).

    Article Title: NR3C1/GLMN-Mediated FKBP12.6 Ubiquitination Disrupts Calcium Homeostasis and Impairs Mitochondrial Quality Control in Stress-Induced Myocardial Damage.
    Article Snippet: .. The ubiquitination level of FKBP12.6 was assessed by Western blot using a specific anti-FKBP12.6 antibody (1:2000; Proteintech, 15114-1-AP). .. All data were analyzed using GraphPad Prism (version 9).



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    Image Search Results


    Effect of CHSSC on the interaction between FKBP12.6 and RyR2 in ischemic H9C2 cells. (a) Co-immunoprecipitation was used to evaluate the interaction between FKBP12.6 and RyR2. (b) Quantitative analysis of the level of RyR2/FKBP12.6 in each group. N = 3 samples per group. All data are expressed as means ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. the control + H-89 group; # P < 0.05, ## P < 0.05, ### P < 0.001 vs. the model + H-89 group.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Chai-Hu-San-Shen Capsule Ameliorates Ventricular Arrhythmia Through Inhibition of the CaMKII/FKBP12.6/RyR2/Ca 2+ Signaling Pathway in Rats with Myocardial Ischemia

    doi: 10.1155/2022/2670473

    Figure Lengend Snippet: Effect of CHSSC on the interaction between FKBP12.6 and RyR2 in ischemic H9C2 cells. (a) Co-immunoprecipitation was used to evaluate the interaction between FKBP12.6 and RyR2. (b) Quantitative analysis of the level of RyR2/FKBP12.6 in each group. N = 3 samples per group. All data are expressed as means ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. the control + H-89 group; # P < 0.05, ## P < 0.05, ### P < 0.001 vs. the model + H-89 group.

    Article Snippet: Anti-FKBP12.6 (#sc-376135) was from Santa Cruz Biotechnology (Shanghai, China).

    Techniques: Immunoprecipitation